Enable DNA Sequencing | Biosensor Integration | Hochuen

To facilitates DNA sequencing, the biosensor integration techonology of Hochuen is tailored to provide the stable carrier for DNA sequencing process.

DNA Sequencing And Its Importance

DNA Sequencing is a mandatory process in DNA to figure the order in which the DNA will be sequenced. Hochuen is an institution that researches these matters and creates related technologies.

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A common phenomenon in a piece of DNA is the DNA sequencing process. It is the method that sequences the nucleotides of DNA in 4 different bases. They are As, Ts, Cs, and Gs. In this process, there are many ways that you can approach it and get various advantages. It is a time-consuming and costly project that requires accurate measurements.

That is where Hochuen helps with their advanced machinery. They excel at creating some of the most creative techs to help you get any accurate result when working with DNA sequencing. Thanks to their long efforts, now DNA sequencing is an easy and straightforward matter to perform. It also takes lesser times and costs lower using the techs from Hochuen.

What is DNA Sequencing?

In DNA Sequencing, people go through some steps to make the sequencing order of the nucleotide bases. Keep in mind that it was an expensive and effortful task back in the day. Since science and technologies have advanced so much, now it is not much of a hurdle. But it is not a new process. Sequencing of the DNA has been practiced for a long time. A big part of that is the human genome sequencing project that was completed in 2003.

Although we put them on the same topic, genome sequencing and DNA sequencing are not the same processes. Sequencing a Genome is a far more complex thing than just a piece of DNA. Even today, it is a pretty difficult task to perform.

Genome is every DNA that a species own. Sequencing the genome requires us to divide the DNA and sequence every piece separately. Finally, all the sequences will be put in a consensus.

What is Sanger Sequencing?

One of the most popular and used DNA sequencing methods is the Sanger sequencing. In this system, a part of DNA will be sequenced regularly. Each fraction of DNA will have a length of 900 base pairs. Hence, this method is also known as the Chain Termination Method. This system was also used in human genome sequencing.

However, during the human genome sequencing, the regions of DNA were not 900 base pairs long. They were so much longer. Hence, the researchers had to run multiple Sanger sequencing on each part to determine the sequence of the whole DNA. After several small fragments of DNA are done being sequenced, they are assembled and overlapped on each other. Thus, the researchers can get a whole picture of a bigger DNA part. By continuing the process, gradually, it is possible to sequence a whole chromosome.

Sanger Sequencing Ingredients

Below are the ingredients needed for a Sanger Sequencing process. They are the same as PCR ingredients.

• A primer. It is a small fragment of DNA, which is single-stranded. It binds with the DNA template.
• A polymerase enzyme of DNA.
• The DNA template.
• The DNA nucleotides. There are four of them.
• Dideoxy of the four nucleotides.

Aside from the Sanger sequencing, there is another DNA sequencing method, which is highly popular nowadays. It is Next-generation sequencing, an advanced project from all angles. It is faster, cheaper, and more accurate. Next-generation sequencing makes it possible to perform multiple DNA part sequencing at the same time. Unlike Sanger sequencing, Next-generation sequencing can read from 50 to 700 base pairs instead of 900.

Since faster sequencing is a way to gain more insights into a genome at a low cost, it opens many possibilities. Hochuen is using this idea to make it even more practical.